DNA Script ssDNA oligos-1

Meet our revolutionary Enzymatic DNA Synthesis platform

Solid Supports and iDNA

Access previously out-of-reach sequences

Avoid rejection and the need to redesign, our platform can accommodate your challenging projects including poly-A tails, telomeric sequences, ITRs and sequences with secondary structure.  

TdT DNA Synthesis

Length no longer a limitation

Our groundbreaking EDS technology enables you to order oligos up to 500 nucleotides in length without the need to stitch together.

Natural DNA-2

Benefit from natural DNA

Not only is Enzymatic DNA Synthesis more efficient, but it also has a much lower environmental impact than traditional DNA synthesis.

“Enzymatic DNA synthesis (EDS) has the potential to be faster and give higher-purity DNA with none of the drawbacks of the conventional phosphoramidite DNA synthesis method”

Science Advances, vol 9, Issue 27

How EDS works

 1. Engineered Terminal Deoxynucleotidyl Transferase (TdT) adds reversibly-terminated nucleotides to the 3’-end of any single DNA strand with high fidelity and coupling efficiency.

 2. Reversibly terminated nucleotide triphosphates (dNTPs) pause synthesis after a single base addition. These dNTPs are relatively small and rapidly and are efficiently restored using mild acidic conditions. They leave no scars, yielding completely natural, molecular biology-ready DNA.

 3. A solid support allows for the control of synthesis scale and manipulating the growing oligo strands throughout the EDS process. The solid support has physical and chemical features optimized to enable the synthesis of ready-to-use long and complex DNA.

Capability

dna-1

Long Oligos from 15 nt to 500 nt
• High accuracy of only 0.35% error rate
• 50 pmol delivered ready to use in ultrapure water

drop_orange-1

More Environmentally Friendly
• Large reduction in hazardous  chemical use
• More sustainable

dna_orange-1

Complex DNA Designs
• High GC content, poly-A, ITR, UTR, promoters etc.
• No need to redesign to remove complexity

clock_orange-1

Reliability
• Typically ships within 10 days
• Clear communication on delivery timelines

Be one of the first to test the limits of our new ssDNA 

Our EDS-powered single-stranded DNA (ssDNA) is now ready to order in private Beta. If you’d like to be one of the first to see how far our oligo production capabilities can go:

1. Check your oligo sequences in our tool via the button below

2. Download the request form generated by the tool

3. Send it us to using the form on the right. We’ll reply to your request within 24 working hours.